Dr. Hans Guldner, Redaktion Wissenschaft, Internet
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Wissenschaft


WISSENSCHAFT / FORSCHUNG SCHWERPUNKTE (bis 1996):

  • Autoimmunkrankheit Systemischer Lupus erythematodes: Molekularbiologische Identifizierung der Sm- und RNP-Antigene
    (Institut für Molekulare Genetik, Universität Heidelberg).

  • Autoantikörper bei entzündlichen rheumatischen Erkrankungen: Identifizierung und Charakterisierung von Zellkernantigenen
    (Institut für Molekulare Genetik, Universität Heidelberg).

  • Autoimmun-Antigene: Identifizierung, Klonierung und molekularbiologische Charakterisierung
    (Max-Planck-Institut für Biochemie in Martinsried).

  • Identifizierung und Charakterisierung von Autoimmun-Antigenen; molekularbiologische Charakterisierung von neuartigen Strukturen des Zellkerns; Nuclear Dots, Sp100-Protein und Splice-Varianten, Dynamik von Nuclear Dots durch exogene Faktoren wie Interferon, Viren, Stress
    (Max-Planck-Institut für Biochemie in Martinsried und Heinrich-Pette-Institut für Virologie in Hamburg).



PUBLIKATIONSVERZEICHNIS WISSENSCHAFT

   Originalpublikationen

  1. Hügle, B., H.H. Guldner, F.A. Bautz, and A. Alonso. 1982. Crossreaction of hnRNP-proteins of HeLa cells with nuclear proteins of Drosophila melanogaster demonstrated by a monoclonal antibody. Exp. Cell Res. 142:119-126.

  2. Guldner, H.H., H.-J. Lakomek, and F.A. Bautz. 1983. Identification of human Sm and (U1)RNP antigens by immunoblotting. J. Immunol. Methods 64: 45-59.

  3. Guldner, H.H., H.-J. Lakomek, and F.A. Bautz. 1984. Human anti- centromere sera recognise a 19.5 kD nonhistone chromosomal protein from HeLa cells. Clin. Exp. Immunol. 58:13-20.

  4. Guldner, H.H., H.-J. Lakomek, C. Szostecki, and F.A. Bautz. 1985. Autoantibody-profiles in patients with SLE, scleroderma and overlap-syndromes. In : Protides of the Biological Fluids (H. Peeters ed.) 33:287-291. Pergamon Press Oxford.

  5. Guldner, H.H., H.-J. Lakomek, and F.A. Bautz. 1986. Anti-(U1) RNP and anti-Sm autoantibody profiles in patients with systemic rheumatic diseases: differential detection of immunoglobulin G and M by immunoblotting. Clin. Immunol. Immunopathol. 40:532-538.

  6. Guldner, H.H., C. Szostecki, H.-P. Vosberg, H.-J. Lakomek, E. Penner, and F.A. Bautz. 1986. Scl70 autoantibodies from scleroderma patients recognize a 95 kDa protein identified as DNA topoisomerase I. Chromosoma 94:132-138.

  7. Lakomek, H.-J., H.H. Guldner, F.A. Bautz, G. Goerz, P. Kind, H. Mensing, und H.L. Krüskemper. 1987. Kernantikörper als serologische Marker der progressiv systemischen Sklerodermie (PSS). Der Hautarzt 38:63-69.

  8. Netter, H.J., H.H. Guldner, C. Szostecki, H.-J. Lakomek, and H. Will. 1988. A recombinant autoantigen derived from the human (U1) small nuclear RNP-specific 68 kD protein. Expression in Escherichia coli and serodiagnostic application. Arthritis Rheum. 31:616-621.

  9. Guldner, H.H., H.J. Netter, C. Szostecki, H.-J. Lakomek, and H. Will. 1988. Epitope mapping with a recombinant human 68-kDa (U1) ribonucleoprotein antigen reveals heterogeneous autoantibody profiles in human autoimmune sera. J. Immunol. 141:469-475.

  10. Guldner, H.H., H.J. Netter, C. Szostecki, E. Jaeger, and H. Will. 1990. Human anti-p68 autoantibodies recognize a common epitope of U1 RNA containing small nuclear ribonucleoprotein and influenza B. J. Exp. Med. 171:819-829.

  11. Netter, H.J., H.H. Guldner, C. Szostecki, and H. Will. 1990. Major autoantigenic sites of the (U1) small nuclear ribonucleoprotein-specific 68-kDa protein. Scand. J. Immunol. 32:163-176.

  12. Szostecki, C., H.H. Guldner, H.J. Netter, and H. Will. 1990. Isolation and characterization of cDNA encoding a human nuclear antigen predominantly recognized by autoantibodies from patients with primary biliary cirrhosis. J. Immunol. 145:4338-4347.

  13. Netter, H.J., H. Will, C. Szostecki, and H.H. Guldner. 1991. Repetitive p68-autoantigen specific epitopes recognized by human anti-(U1) small nuclear ribonucleoprotein autoantibodies. J. Autoimmunity 4:651-663.

  14. Szostecki, C., H. Will, H.J. Netter, and H.H. Guldner. 1992. Autoantibodies to the nuclear Sp-100 protein in primary biliary cirrhosis and associated diseases: epitope specificity and immunoglobulin class distribution. Scand. J. Immunol. 36:555-564.

  15. Guldner, H.H., C. Szostecki, T. Grötzinger, and H. Will. 1992. IFN enhance expression of Sp100, an autoantigen in primary biliary cirrhosis. J. Immunol. 149:4067-4073.

  16. Maul, G.G., H.H. Guldner, and J.G. Spivack. 1993. Modification of discrete nuclear domains induced by herpes simplex virus type I immediate early gene 1 product (ICP0). J. Gen. Virol. 74:2679-2690.

  17. Sternsdorf, T., H.H. Guldner, C. Szostecki, T. Grötzinger, and H. Will. 1995. Two nuclear dot-associated proteins, PML and Sp100, are often co-autoimmunogenic in patients with primary biliary cirrhosis. Scand. J. Immunol. 42:257-268.

  18. Grötzinger, T., K. Jensen, H.H. Guldner, T. Sternsdorf, C. Szostecki, M. Schwab, L. Savelyeva, B. Reich, and H. Will. 1996. A highly amplified mouse gene is homologous to the human interferon-responsive Sp100 gene encoding an autoantigen associated with nuclear dots. Mol. Cell. Biol. 16:1150- 1156.

  19. Guldner, H.H., C. Szostecki, P. Schröder, U. Matschl, K. Jensen, C. Lüders, H. Will, and T. Sternsdorf. 1999. Splice variants of the nuclear dot-associated Sp100 protein contain homologies to HMG-1 and a human nuclear phosphoprotein-box motif. J. Cell Sci. 112, 733-747.

   Übersichtsartikel

  1. Guldner, H.H. 1992. Mapping of epitopes recognized by anti-(U1)RNP autoantibodies. Mol. Biol. Rep. 16:155-164.

  2. Szostecki, C., H. Will, und H.H. Guldner. 1994. Die humorale Autoimmunantwort gegen das nukleäre Sp100 Autoantigen in Patienten mit primär biliärer Zirrhose. Z. ärztl. Fortbild. 88: 573-578.

  3. Szostecki, C., H.H. Guldner, T. Sternsdorf und H. Will. 1997. Autoantikörper gegen `Nuclear Dots´ bei der Primär biliären Zirrhose. In: Autoantikörper (Ed. K. Conrad), Wolfgang Pabst Publishers Lengerich, Berlin, Wien, Zagreb. 492-509.

  4. Szostecki, C., H.H. Guldner, H. Will. 1997. Autoantibodies against „nuclear dots“ in primary biliary cirrhosis. Semin. Liver Dis.17: 71-78.

   Kurzmitteilungen

  1. Netter, H.J., H.H. Guldner, C. Szostecki, H.-J. Lakomek, and H. Will. 1987. Isolation and serodiagnostic application of a genetically engineered small nuclear ribonucleoprotein antigen. Immunobiology 175:346.

  2. Netter, H.J., H.H. Guldner, C. Szostecki, H.-J. Lakomek, and H. Will. 1987. Identification and serodiagnostic applications of a genetically engineered snRNP antigen. Z. Rheumatol. 46:281.

  3. Kalla, M., M. Kutzer, H.H. Guldner, K. Decken, G. Goerz und H.-J. Lakomek. 1988. Ein Dot-Spot-Test mit DNS-Topoisomerase I als sensitives Nachweissystem des Scl-70-Antikörpers. Z. Rheumatol. 47:253.

  4. Guldner, H. 1988. Heterogeneous autoantibody profiles in human autoimmune sera as revealed by epitope mapping with a recombinant human 68 kDa (U1) RNP-antigen. Z. Rheumatol. 47:313.

  5. Guldner, H.H., H.J. Netter, C. Szostecki, H.-J. Lakomek, and H. Will. 1988. Heterogeneity of autoantigenic epitopes on a recombinant human 68 kDa (U1) RNP-antigen as revealed by epitope mapping. Immunobiology 178:47.

  6. Guldner, H.H., H.J. Netter, C. Szostecki, E. Jaeger, and H. Will. 1989. Molecular mimicry: A common epitope of the (U1)snRNP associated p68 autoantigen and a protein of a human pathogenic virus. In: Molecular and Cell Biology of Autoantibodies and Autoimmunity (Bautz, Kalden, Homma & Tan eds.). Springer-Verlag Berlin, Heidelberg, New York; p. 22.

  7. Netter, H.J., H.H. Guldner, C. Szostecki, H.-J. Lakomek, and H. Will. 1989. Identification of several independent autoreactive epitopes of the human 68 kDa (U1)snRNP-autoantigen. In: Molecular and Cell Biology of Autoantibodies and Autoimmunity (Bautz, Kalden, Homma & Tan eds.). Springer-Verlag Berlin, Heidelberg, New York; p. 60.

  8. Netter, H.J., C. Szostecki, H. Will, and H.H. Guldner. 1990. Mapping of multiple autoepitopes on the p68 (U1)RNP-antigen and identification of a determinant shared with influenza B-viruses. Clin. Rheumatol. 9:101.

  9. Szostecki, C., H.H. Guldner, H.J. Netter, and H. Will. 1990. Detection of autoantibodies against a novel nuclear autoantigen (sp-100) by an ELISA based on recombinant antigen. Clin. Rheumatol. 9:110.

  10. Szostecki, C., H.H. Guldner, H.J. Netter, and H. Will. 1990. High prevalence of autoantibodies to a nuclear antigen in primary biliary cirrhosis: molecular cloning of a major antigenic domain. J. Hepatol. 11: 59 (suppl.).

  11. Guldner, H.H., R. Gschwendner, H.J. Netter, and M. Meurer. 1991. Major autoantigenic sites of U1-RNP antibodies in mixed connective tissue disease (MCTD). J. Invest. Dermatol. 96:1027.

  12. Szostecki, C., H.H. Guldner, H.J. Netter, and H. Will. 1991. Epitope specificity and immunoglobulin isotypes of anti-Sp 100 autoantibodies in PBC and associated diseases. J. Hepatol. 13:74 (suppl.).

  13. Guldner, H.H., C. Szostecki, and H. Will. 1996. The nuclear dot-associated Sp100 protein: characterization of HMG-box containing splice variants. Europ. J. Cell Biol. 69:88 (suppl.).